Ozonation in Wastewater Disinfection
More details
Hide details
Water Purification and Protection Plant, Rzeszow University of Technology, Poland
Doctoral School of the Rzeszow University of Technology, Rzeszow University of Technology, Poland
These authors had equal contribution to this work
Submission date: 2023-10-24
Final revision date: 2023-11-14
Acceptance date: 2023-11-23
Online publication date: 2023-12-14
Publication date: 2023-12-14
Corresponding author
Izabela Kiełb-Sotkiewicz   

Doctoral School of the Rzeszow University of Technology, Rzeszow University of Technology, Powstańców Warszawy, 35-959, Rzeszów, Poland
Civil and Environmental Engineering Reports 2023;33(3):63-75
Due to the potential microbiological hazard associated with discharging treated sewage into the receiving body, its disinfection is a key issue to protect ecological safety and human health. Water scarcity and drinking water supply, irrigation, rapid industrialization, use of treated water, protection of water sources, overpopulation and environmental protection force us to look for solutions to ensure safe reuse of wastewater, and this depends primarily on the quality of wastewater disinfection. Many wastewater disinfection methods are commonly used. One of the chemical processes of disinfection sludge is ozonation. Ozonation is widely used in wastewater treatment by oxidation, because ozone is a very strong and effective oxidizing agent. Studies have shown that the effectiveness of ozone in disinfecting water and sewage is up to 50% greater than that of chlorine . An additional advantage of this method is that it also eliminates odors that may be unavailable. The article presents the results of research on the effectiveness of ozonation treatment in the disinfection of treated sewage, based on indicator bacteria such as coliforms, including Escherichia coli, mesophiles, psychrophiles, and spores. The study took into account various effects of time (dose) and temperature. For the purpose of this study, both traditional and modern methods of assessing microbiological quality of wastewater were used. The first one is represented by conventional culture measurements and the second one by using a luminometer (ATP) and flow cytometer (FCM).
Amin, MM, Hashemi, H, Bovini, AM and Hung, YT 2013. A review on wastewater disinfection. International Journal of Environmental Health Engineering 2, 1-9.
Sobczak, P 2021. [Disinfection process of treated wastewater using ozone]. [Water Technology] 1, 32-35.
Blue Planet, [Wastewater ozonation], daily access:25.09.2023r.
Stasik, D 2020. [Drought or flood? 5 smart solutions for water management in the city] https://dariuszstasik.com/susz... (daily access: 17.10.2023r.).
Płuciennik-Koropczuk, E, Jakubaszek, A 2012. [Susceptibility of sewage to biochemical decomposition in mechanical-biological treatment processes]. [Science Notebooks] 148, 73-83.
Grudziński, M, Pietruszka, A and Sawicki, W 2015. Anaerobic digestion in sanitization of pig slurry and biomass in agricultural biogas plant. Journal of Microbiology, Biotechnology and Food Sciences 5, 524-526.
Rizzo, L, Manaia, C, Merlin, C, Schwartz, T, Dagot, C and Ploy, MC 2013. Urban wastewater treatment plants as hotspots for antibiotic resistant bacteria and genes spread into the environment: a review. Science of The Total Environment Journal 447, 345–360.
Jawecki, B, Marszałek, J, Pawęska, K, Sobota, M and Malczewska, B 2016. [Construction and operation of home sewage treatment plants in the light of applicable regulations]. [Infrastructure and Ecology of Rural Areas] 2, 501-516.
Hammes, FA, Egli, T 2005. New method for assimilable organic carbon determination using flow-cytometric enumeration and a natural microbial consortium as inoculum. Environmental Science & Technology 39, 3289–3294.
Yunho, L, Imminger, S, Czekalski, N, Gunten, U and Hammes, F 2016. Inactivation efficiency of Escherichia coli and autochthonous bacteria during ozonation of municipal wastewater effluents quantified with flow cytometry and adenosine tri-phosphate analyses. Water Research 101, 617-627.
Biń, AK 1999. [The use of ozonation and advanced oxidation processes for drinking water treatment and sewage treatment in Poland]. [Environmental Protection Yearbook] 1, 7-25.
Olańczuk-Neyman, K, Quant, B 2015. [Wastewater disinfection].Warszawa: Seidel-Przywecki Sp. z o.o.
Konieczny, K and Ćwikła, J 2014. [The use of reclaimed water after microfiltration cleaning]. [Water Supply, Water Quality And Protection] 1, 641-648.
Heß, S and Gallert, C 2015. Sensitivity of antibiotic resistant and antibiotic susceptible Escherichia coli, Enterococcus and Staphylococcus strains against ozone. Journal of Water and Health 13,1020–1028.
Martinelli, M, Giovannangeli, F, Rotunno, S, Trombetta, CM and Montomoli, E 2017. Water and air ozone treatment as an alternative sanitizing technology. Journal of Preventive Medicine and Hygiene 58, 48–52.
Natonek, J 2022. [The use of ozone in disinfection and increasing the operational safety of the water supply network], Doctoral Dissertation, Silesian University of Technology, Faculty of Environmental Engineering and Energy. Scientific discipline: Environmental Engineering, Mining and Energy.
Xu, P, Janex, ML, Savoye, P, Cockx, A and Lazarova, V 2002. Wastewater disinfection by ozone: main parameters for process design. Water Research 36, 1043–1055.
Gehr, R, Wagner, M, Veerasubramanian, P and Payment, P 2003. Disinfection efficiency of peracetic acid, UV and ozone after enhanced primary treatment of municipal wastewater. Water Research 37, 4573–4586.
Lüddeke, F, Heß, S, Gallert, C, Winter, J, Güde, H and Löffler, H 2015. Removal of total and antibiotic-resistant bacteria in advanced wastewater treatment by ozonation in combination with different filtering techniques. Water Research 69, 243–251.
Dodd, MC 2012. Potential impacts of disinfection processes on elimination and deactivation of antibiotic resistance genes during water and wastewater treatmen., Journal of Environmental Monitoring 14, 1754–1771.
Janex, ML, Savoye, P, Roustan, M, Do-Qunag, Z, Laine, JM and Lazarowa, V 2008. Wastewater Disinfection by Ozone: Influence of Water Quality and Kinetics Modeling, Ozon., Science & Engineering, 22, 113-121.
Bader, H 2008. Determination of Ozone In Water By The Indigo Method: A Submitted Standard Method, A Submitted Standard Method, Ozone. Science & Engineering, 4, 169-176.
Rekhate, C, Srivastava, JK 2020. Recent advances in ozone-based advanced oxidation processes for treatment of wastewater- A review. Chemical Engineering Journal Advances 3, 100031.
Tripathi, S, Hussain, T 2022. Water and Wastewater Treatment through Ozone-based technologies. In: Maulin S.(ed) Development in Wastewater Treatment Research and Processes Removal of Emerging Contaminants from Wastewater Through Bio-nanotechnology, OpenAcess ELSEVIER 139-172.
Webside: www.velpol.org.pl; (daily access: 15.10.2023r.).
Kunicki-Goldfinger, W 2001. [Bacterial life].Warszawa. PWN.
Emerick, RW, Loge, FJ, Ginn, T and Darby, JL 2000. Modeling the inactivation of particle-associated coliform bacteria. Water Environment Research 72, 432-438.
Ramseier, MK, Gunten, U, Freihofer, P and Hammes, F 2011. Kinetics of membrane damage to high (HNA) and low (LNA) nucleic acid bacterial clusters in drinking water by ozone, chlorine, chlorine dioxide, monochloramine, ferrate(VI), and permanganate. Water Research 45, 1490-1500.
Cao, H, Wang, J, Kim, JH, Guo, Z, Xiao, J, Yang, J, Chang, J, Shi, Y and Xie, Y 2021. Different roles of Fe atoms and nanoparticles on g-C3N4 in regulating the reductive activation of ozone under visible light. Applied Catalysis B: Environmental Journal 296, 120362.
Pavon-Dominguez, P, Plocoste, T 2021. Coupled multifractal methods to reveal changes in nitrogen dioxide and tropospheric ozone concentrations during the COVID-19 lockdown, Atmospheric Research 261, 105755.
Hao, Y et all 2021. Unprecedented decline in summertime surface ozone over eastern China in 2020 comparably attributable to anthropogenic emission reductions and meteorology. Environmental Research Letters 16, 124069.
Quant, B et all 2014. [Possibilities of disinfection of biologically treated sewage on the example of the "Wschód" sewage treatment plant in Gdańsk”], [Marine Engineering and Geotechnology] 5, 397-406.
Bergel, T [Chlorine or ozone - a few words about wastewater disinfection...] Chlor_czy_ozon_-_dezynfekcja_sciekow.pdf (ozonowanie.pl) daily access: 17.10.2023r.).
Hussain, K, Khan, N, Vambol, V, Vambol, S, Yeremenko, S and Syderenko, V 2022. Advancement in Ozone base wastewater treatment technologies: Brief review. Ecological Questions 33, 7–19.
Dymaczewski, Z, Oleszkiewicz, JA, Sozański, MM 1997.[Sewage treatment plant operator's guide]. Poznań. PZITS.
Kasuga, I, Nakamura, H, Kurisu, F and Furumal, H 2021. Characterization of microbial regrowth potential shaped by advanced drinking water treatment. H2Open Journal, 4, 157–166.
Journals System - logo
Scroll to top